Research Technician/Analyst/Scientist to work on protein engineering/synthetic biology
We are looking for a biochemist/molecular biologist/structural biologist with a background in protein chemistry to work in the Department of Biochemistry at Duke University Medical Center in Durham, North Carolina. You will be part of a small group that is developing fluorescent biosensors based on engineered proteins for a variety of biomedical applications. We use a range of experimental techniques including synthetic gene construction, heterologous protein expression, protein purification by affinity chromatography, different fluorophore labeling strategies, a range of methods to analyze biosensor function and stability. If you are interested, please send us a resume describing your training and professional employment history.
The responsibilities for this position is to assist with laboratory procedures and techniques such as:
- Heterologous protein expression in E.coli
- Protein purification by affinity chromatography
- Protein gel electrophoresis
- Biophysical protein characterization and ligand titrations
- General laboratory maintenance: e.g., preparations of buffers and reagents, autoclave plastic/glassware/solutions, maintain laboratory equipment
- Ordering of chemicals and supplies
- Protein purification and protein chemistry
- Excellent organizational skills and attention to details
- Meticulous record keeping
- Good computer skills; Microsoft Word, Excel, OneNote
- Excellent written and verbal communication skills
- Work well with others
Desired other skills:
Chromatography using the ÄKTA system, and molecular biology (cloning).
A minimum of a Bachelor’s degree in Molecular biology, Biochemistry, or a related discipline.
At least one-year full-time laboratory research experience in a biotech/pharmaceutical company or academic research environment.